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Purpose of BatchKi
The software BatchKi is designed for automatic determination of enzyme inhibition constants (either ``tight-binding'' or ``classical'') from kinetic data that are usually obtained by using plate-reader hardware. In addition to the apparent inhibition constants, the program also reports 
values. Optionally, enzyme concentrations can be determined simultaneously with the determination of inhibition constants.
BatchKi can use as input data either complete reaction progress curves (from continuous enzyme assays), or single-point data obtained for example in radioactive kinase assays and other end-point measurements.
BatchKi has been successfully implemented also in the analysis of dose-response data from cell-based assays, as opposed to assays utilizing purified enzymes. In the case of cell-based assays, the software utilizes the four-parameter logistic equation as a fitting model.
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Petr Kuzmic | Jul 12 2008