This page refers to a
rational approach to the design of dilution series, used in dose-response screening of enzyme inhibitors.
The method is equally applicable to both "tight binding" and "classical" (weakly bound) enzyme inhibitors.
Includes a freely accessible software tool to automatically generate dilution series based on the range of
inhibition constant values for inhibitors in the screening pool.
PDF Preprint of a manuscript describing the method
This manuscript describes an application of the Box-Lucas theory to the design of inhibitor dilution series.
This online form performs the Box-Lucas calculations described in the above manuscript.
NOTE: The Merge Factor in the calculator represents the maximum ratio of two inhibitor
concentrations that should be treated as uniquely different in the design table. Values closer to unity
will produce a greater number of inhibitor concentrations in the final, optimized dilution series.
For details, see the attached preprint.
www.biokin.com/design/screen/index.html Sat May 28 13:14:18 2011
