This section of the DynaFit website contains tutorials explaining the use of the program on real-world examples taken from the biochemical literature.

Rev-Responsive Element of HIV-1

The purpose of this experiment [Lacourciere, K.A., et al. (2000) Biochemistry 39, 5630] was to measure the strength of non-covalent binding (i.e., the equilibrium dissociation constant) between two biological macromolecules. One of them is the Rev-responsive element (RRE) of the Human Immunodeficiency Virus, and the other is the Rev protein, which regulates viral replication. RRE was first chemically modified by the introduction of fluorescent label. Fluorescence was then monitored at a fixed concentration of RRE (30 nM) while the concentration of a peptide similar to Rev was varied (0 - 300 nM). The dissociation constant for the RRE-Rev complex was estimated quite accurately from the fluorescence intensities observed at equilibrium.

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