Below are a few examples that were used to test the present implementation of the King-Altman method. You may wish to modify the examples, for example by adding activators and/or inhibitors.
Copy text from the editable areas below, and paste it into the data submision page.
IMPORTANT NOTE: All activators and/or inhibitors must be shown in the [modifiers] section (as a comma separate list, if more than one modifier is present).
Irreversible Michaelis-Menten mechanism
Reversible Michaelis-Menten mechanism
Partial mixed-type noncompetitive inhibition
Botts Morales modifier mechanism
Bi Bi Ordered mechanism
Bi Bi Theorell-Chance mechanism
Bi Uni Random mechanism
Bi Bi Random mechanism
Mixed-type inhibition of dihydrofolate reductase
|Irreversible Michaelis-Menten mechanism|
The simplest possible enzymatic reaction mechanism:
|Reversible Michaelis-Menten mechanism|
Fully reversible version of the mechanism above:
Reversible Michaelis-Menten in the presence of a competitive inhibitor:
|Partial mixed-type noncompetitive inhibition|
The ternary complex Enzyme-Substrate-Inhibitor is partially catalytically active:
|Botts Morales modifier mechanism|
The Botts-Morales general modifier mechanism is a favored system for testing rate equation derivation methods:
|Bi Bi Ordered mechanism|
Compare the results with Segel's Enzyme Kinetics, page 562 (Equation IX-87):
|Bi Bi Theorell-Chance mechanism|
Compare the results with Segel's Enzyme Kinetics, page 594 (Equation IX-122):
|Bi Uni Random mechanism|
Compare the results with Segel's Enzyme Kinetics, page 647 (Equation IX-181):
|Bi Bi Random mechanism|
Compare the results with Segel's Enzyme Kinetics, page 649: this mechanism should produce 48 denominator terms.
Benkovic et al. (1988) painstakingly determined all elementary rate constants in this relatively complex mechanism:
|Mixed-type inhibition of dihydrofolate reductase|
Appleman et al. (1988) determined that certain inhibitors bind both to free dihydrofolate reductase, and to the DHRF-NADPH+ complex.
This system represents the most complex mechanism in this collection of example problems: