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"Mechanical effects on the kinetics of the HIV proteinase deactivation." Kuzmic, P.; Peranteau, A.G.; Garcia-Echeverria, G.; and Rich, D.H. (1996) Biochem. Biophys. Res. Commun. 221, 313-7. Abstract: The proteinase from HIV undergoes rapid and irreversible deactivation caused by mild mechanical stirring. Both the free enzyme and the ternary Michaelis complex disappear in two separate first-order processes, with half-times of 3.0 and 0.8 minutes, respectively. Ignoring these deactivation steps distorts the results of kinetic analyses.

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