1 2 3 4 5 6 7 8 9 10 11 12 13 14 15 16 17 18

ATG AAA ACG CTC TAC TTG ATT CCT ATC TGG TTA TTC CTC ATT TCG CAA TGC AGT

Met Lys Thr Leu Tyr Leu Ile Pro Ile Trp Leu Phe Leu Ile Ser Gln Cys Ser

19 20 21 22 23 24 25 26 27 28 29 30 31 32 33 34 35 36

GGA AAA TCT GTG AAG AAA TGT GGT CTC AAT GAA AGG TTG GAC TGT GGT AAT CTA

Gly Lys Ser Val Lys Lys Cys Gly Leu Asn Glu Arg Leu Asp Cys Gly Asn Leu

37 38 39 40 41 42 43 44 45 46 47 48 49 50 51 52 53 54

AAG GCA TGT GAG CGC AAA TGC AAT GAG GAG GAA AGC GAG GAA GAA ATT GAG GCG

Lys Ala Cys Glu Arg Lys Cys Asn Glu Glu Glu Ser Glu Glu Glu Ile Glu Ala

55 56 57 58 59 60 61 62 63 64 65 66 67 68 69 70 71 72

AGA TGC CTC TCA CGT GAT TGT CTT GGT CGT GTT TGC ATG TGC GAT TAC GGA TTC

Arg Cys Leu Ser Arg Asp Cys Leu Gly Arg Val Cys Met Cys Asp Tyr Gly Phe

73 74 75 76 77 78 79 80 81 82 83 84 85 86 87 88 89 90

TAC AGG AAC AAA GAC GGT GAA TGT GTG ACA GAA GAT GAC TGC GAG TAT GAT AAC

Tyr Arg Asn Lys Asp Gly Glu Cys Val Thr Glu Asp Asp Cys Glu Tyr Asp Asn

91 92 93 94 95 96 97 98 99 100 101 102 *

ATG GAG ATT ATT ACT TTC CCA CCA GAA TCC AAA CAC TGA CCG GTA ATT CCA ACT

Met Glu Ile Ile Thr Phe Pro Pro Glu Ser Lys His stop

CTC GAA CCC CAA TCG AAT GAT CCA ATG CTT CAC TGG CTT CCC TTC ATG TTA GTA

GCT TTG CTT GAT TCT GTG TAT TTG GGC ACT GTC TAG TGA TGA GGA AAG TAA AGC

ATT TCA ACA AAA AAA AAA AAA

Figure 1. Nucleotide and translated amino acid sequence of AceAP1 cDNA: The open reading frame of the AceAP1 cDNA consists of 432 nucleotides from the initial ATG to the 3' polyadenylation tail. The translated amino acid sequence of AceAP1 includes a putative 18 amino acid secretory signal sequence, followed by a mature protein of 84 amino acids. The arrow denotes the signal sequence cleavage site predicted using the computer software program SignalP. The 10 cysteine residues are in bold. The predicted molecular weight of the mature AceAP1 protein is 9626 Da. These sequence data are available from GenBank/EMBL/DDBJ under accession number AF399710.