Figure 3. The anticoagulants rAceAP1 and rAcAP5 are immunologically distinct. Recombinant hookworm factor Xa inhibitors were subjected to SDS-PAGE, followed by transfer to nitrocellulose. The blots were probed with polyclonal rabbit IgG raised against rAceAP1 (left panel) or rAcAP5 (center panel), followed by a HRP-labeled secondary antibody and detection with chemiluminescent substrate. Lane A: rAceAP1; Lane B: rAcAP5; Lane C: Molecular weight markers. Panel on right shows identical gel stained with Coomassie.