Figure 1

Layout of inhibitor concentrations in the Monte-Carlo simulation study. Dose-response curves labeled a through h correspond to the simulated values of the apparent inhibition constants K = 100 μM (curve a) and K = 10 pM (curve h), stepping by a factor of 10. Symbols represent the reaction velocities at corresponding inhibitor concentrations (eight concentrations per dose-response curve), simulated by using the Morrison equation (1) [1]. To each ideal data point shown here, normally distributed pseudo-random error was added with standard deviation 0.05 (5%). Ten thousand random data sets were generated for each ideal dose-response curve shown here. The resulting "noisy" data were subjected to the weighted-average procedure described in the Methods to determine the apparent inhibition constant K.