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Supplementary Material: AB99-0564
P. Kuzmic et al. (2000) Anal. Biochem., 281, 62-67.

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Numerical data used in the construction of Figure 2 Figure 2 . Column 1 contains the concentration (nM) of a "tight-binding" tryptase inhibitor APC-1390. Column 2 contains the initial reaction velocity in 106 × absorbance units per second.

[I],nM	V
0.00	78.50
0.23	31.90
0.35	21.60
0.53	13.90
0.79	 9.55
1.19	 8.42
1.77	 4.05
4.00	 3.17

Numerical data used in the construction of Figure 3 Figure 3 . Column 1 contains the concentration (µM) of a "classical" (non-tight-binding) tryptase inhibitor phenylguanidine. Column 2 contains the initial reaction velocity in 106 × absorbance units per second.

[I],µM	V
0.00	702.80
0.46	715.60
1.37	722.10
4.12	662.10
12.35	587.20
37.04	454.50
111.10	241.80
333.30	103.30
1000.00	 38.19


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BioKin, Ltd., offers a complete data analysis package for high throughput screening of enzyme inhibitors using the algorithm described in this paper. Visit the BatchKi BatchKi home page to learn more.
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Fri Jan 29 13:19:38 2010