Figure 1

A typical simulation / regression sequence from a Monte-Carlo study of Equation (4), using [E]₀ = 1.0 nM. Synthetic data points were generated at [I]₀ = 1, 0.5, 0.25, ..., 0.0078125, and 0 nM. Pseudo-random error with standard deviation of 3% relative to the generated value was superimposed on the ideal data, and the resulting data set was fit to Equation (4). Ten thousand such simulation / regression runs were performed for each combination of inhibition constants K_T, K_C, and the molar fraction of tight binding impurity, a.