Figure 2

Summary of three typical Monte-Carlo simulations, all conducted at [E]₀ = 1.0 nM and the molar fraction of impurity a = 1%. Upper panel (A): Simulated values were K_C = 0.1 µM, K_T = 0.1 nM, with the pseudo-random error 1%. Middle panel (B): Same as A, except for pseudo-random error 2%. Lower panel (C): Simulated values K_C = 1.0 µM, K_T = 1.0 nM, with the pseudo-random error 1%. For explanation see text. In total, 1302 such Monte-Carlo simulations were performed at 1302 different combinations of K_T, K_C and a (approximately 1.3 million synthetic data sets).