Program PlateKi for Windows-NT/9x/2000/XP is currently used in leading pharmaceutical research laboratories to determine "tight-binding"or "classical" inhibition constants from raw plate-reader data. Associated IC50 values are also determined for compatibility with most corporate databases.
- Automatic model selection in fitting the reaction progress curves (straight line, quadratic parabola, first or second degree exponential).
- Automatic initial estimate of the inhibition constant .
- Optional robust regression with automatic exclusion of outliers .
- Determination of nonsymmetrical confidence intervals for model parameters according to the profile-t method (Bates & Watts, 1988).
- Fitting of dose-response curves either to the Morrison equation for tight binding or to the four-parameter logistic equation.
- High processing speed (approximately 100 milliseconds per inhibition constant, including progress curve analysis).
- Outlier exclusion minimizes labor and removes subjective bias.
- Can be used for secondary in-vitro screening of purified enzymes, or in cell-based assays.
- Summary output file can be imported into Excel and/or corporate databases.
PlateKi for Windows
- Intel® Pentium® III or Celeron® class 800 MHz or faster processor
- Microsoft Windows® XP (SP1) or 2000 (SP2)
- 128 MB RAM
- 20 MB Hard Disk Space
- Ethernet Network Interface Card required for license activation(1)
- CD/DVD-ROM drive required for software installation(2)
The Network Interface Card is used to compute a unique "Computer ID", tied to a particular PlateKi license. Essentially the "Computer ID" required for license activation is an encrypted Media Access Control (MAC address) associated with the given Network Card.
CD/DVD-ROM is not required if the software is being installed by using the downloadable installer file plateki-install.zip.