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| Software |
| Data analysis tools for scientists |
Program DynaFit
Program BatchKi
Program PlateKi
| Program DynaFit |  |
Program
 DynaFit
[P. Kuzmic (1996) Anal. Biochem 237, 260-273] performs simulations and nonlinear least-squares fit of experimental data obtained in a biochemical, biochemical, pharmacological, or biological laboratory.
Types of Experimental Data
- Reaction progress curves
- The change of a physical signal (e.g., absorbance, fluorescence, conductivity, radioactivity) monitored over time.
- Initial reaction velocities
- Slope of reaction progress curves at time zero, obtained at various initial concentration of various reactants.
- Composition at equilibrium
- Measurement of a certain physical quantity at equilibrium, to extract the values of equilibrium binding constants (or dissociation constants).
Desription of Underlying Mechanism
The reaction mechanism is described in DynaFit by using the usual chemical notation. For example, the classic Michaelis-Menten mechanism for enzymatic catalysis is described by the text below, where the symbols E, S, and ES are chosen arbitrarily:
E + S <==> ES : k1 k2
ES ---> E + P : k3
| Program BatchKi | |
Program
BatchKi
performs automatic determinations of tight-binding enzyme inhibition constants using a recently published computations algorithm [P. Kuzmic et al. (2000) Anal. Biochem. 281, 62-67]. A
reprint
of this journal article is available for download.
| Program PlateKi | |
Whereas BatchKi is a server application (running on a Web server), program
PlateKi
is an equivalent that runs as a stand-alone application under the MS Windows operating system. Both programs are used for automatic determination of enzyme inhibition constants.
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www.biokin.com/software.html
Fri Jan 18 10:00:29 2008 |
