This page refers to a
rational approach to the design of dilution series, used in dose-response
screening of enzyme inhibitors. The method is equally applicable to
both "tight binding" and "classical" (weakly bound) enzyme inhibitors.
Includes a freely accessible software tool to automatically generate dilution
series based on the range of inhibition constant values for inhibitors in
the screening pool.
This online form performs the Box-Lucas calculations described in the above manuscript.
NOTE: The Merge Factor in the calculator represents the
maximum ratio of two inhibitor concentrations that should be
treated as uniquely different in the design table. Values closer to unity
will produce a greater number of inhibitor concentrations in the final,
optimized dilution series.
For details, see the attached article.
www.biokin.com/tools/design.html Sat Apr 20 18:22:32 2019