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| Substrate kinetics from reaction progress |
| DynaFit tutorial |
Determination of substrate kinetic parameters from progress curve data: A DynaFit tutorialBioKin Technical Note TN-2015-05Petr KuzmicBioKin Ltd. Draft - Rev. 1.01 - November 16, 2015 Download PDF
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AbstractThe purpose of this DynaFit tutorial is to present detailed, step-by-step method to determine the substrate kinetic constants from a single well chosen reaction progress curve, which is purposely allowed to achieve full conversion of substrate into product. The results show that the steady-state kinetic values obtained either by the classical initial rate method or by the numerical integration method are identical. The advantage of the numerical method is that it requires only a single kinetic experiment (i.e., a single well chosen substrate concentration), whereas the classic initial rate method relies on multiple experiments performed at a variety of substrate concentrations.Keywordsenzyme kinetics; mathematics; DynaFit; tutorial; progress curve analysisHow to CiteKuzmic, P. (2015) Determination of substrate kinetic parameters from progress curve data, BioKin Technical Note TN-2015-05, BioKin Ltd., Watertown MA, [Online] www.biokin.com/TN/2015/05
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Sat Apr 20 18:22:31 2019