


Integrated MichaelisMenten equation in DynaFit. Part 2. 
Determination of substrate kinetic parameters of 5alphaketosteroid reductase 
Integrated MichaelisMenten equation in DynaFit. 2. Application to 5alphaketosteroid reductase
BioKin Technical Note TN201602
Petr Kuzmic
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Abstract
The DynaFit software package (http://www.biokin.com/dynafit/) allows the user
to specify the theoretical model for an arbitrary bio/chemical mechanism by using a symbolic
rather than mathematical notation. However, for convenience, DynaFit also contains a small
number of builtin, predefined fitting models. This Technical Note describes a family of predefined
algebraic fitting models that describe the timecourse of enzyme reactions conforming to
the classic MichaelisMenten kinetic mechanism. An illustrative example includes previously
published data on the substrate kinetics of 5alphaketosteroid reductase. It is shown that the least
squares fit of a single enzymatic progress curve, observed at an optimally chosen substrate concentration,
results in a KM value that is exactly identical to the value obtained by using the much
laborious and less precise initial rate method.


